Abstract Title

Development of Curcumin Loaded Nanoparticles and Evaluation of Antitumor Effects on Prostate Cancer Cell Lines

Presenter Name

Irin Tanaudommongkon

Abstract

Purpose (a):

Curcumin (CUR) is a low molecular weight, lipophilic, yellow polyphenolic compound of Indian spice turmeric. In recent years, CUR has been shown as an effective antiproliferative agent in many cancer cell lines such as breast cancer and prostate cancer. Due to low water solubility and instability, the formulation of CUR is challenging and this prevents the usage of CUR in anticancer application. Lipid based nanoparticle (NP) delivery system is a promising approach to formulate CUR, in terms of its abilities in formulating lipophilic drugs, improving the drug’s pharmacokinetics and biodistribution, and reducing drug toxicity. The main objective of this study is to develop CUR NPs by optimizing NPs with varying compositions of lipids and surfactants.

Methods (b):

The preparation of nanoparticles was performed by a warm o/w microemulsion system. The drug loading and entrapment efficiency of CUR NPs were measured by using HPLC. Particle size was determined by using photon correlation spectroscopy. The in-vitro cytotoxicity of CUR NPs were performed by using MTT assays in PC3 and DU145 prostate cancer cell lines.

Results (c):

We were able to load CUR into the NPs made of Migloyl 812 and TPGS (1:1, w/w). Particle size was less than 150 nm with polydispersity index95% and drug loading was >5%. CUR NPs were stable for up to 5 months at 4°C and up to 96 hours at 37°C in PBS buffer (pH 7.4) without significant changes in particle sizes. For both sensitive and resistant PC3 and DU145 cell lines, CUR NPs significantly reduced IC50 values over free drug.

Conclusions (d):

We successfully prepare CUR NPs using lipid-based NPs. CUR NPs significantly improved cytotoxicity of CUR in sensitive and resistant prostate cancer cells compared to free CUR.

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Development of Curcumin Loaded Nanoparticles and Evaluation of Antitumor Effects on Prostate Cancer Cell Lines

Purpose (a):

Curcumin (CUR) is a low molecular weight, lipophilic, yellow polyphenolic compound of Indian spice turmeric. In recent years, CUR has been shown as an effective antiproliferative agent in many cancer cell lines such as breast cancer and prostate cancer. Due to low water solubility and instability, the formulation of CUR is challenging and this prevents the usage of CUR in anticancer application. Lipid based nanoparticle (NP) delivery system is a promising approach to formulate CUR, in terms of its abilities in formulating lipophilic drugs, improving the drug’s pharmacokinetics and biodistribution, and reducing drug toxicity. The main objective of this study is to develop CUR NPs by optimizing NPs with varying compositions of lipids and surfactants.

Methods (b):

The preparation of nanoparticles was performed by a warm o/w microemulsion system. The drug loading and entrapment efficiency of CUR NPs were measured by using HPLC. Particle size was determined by using photon correlation spectroscopy. The in-vitro cytotoxicity of CUR NPs were performed by using MTT assays in PC3 and DU145 prostate cancer cell lines.

Results (c):

We were able to load CUR into the NPs made of Migloyl 812 and TPGS (1:1, w/w). Particle size was less than 150 nm with polydispersity index95% and drug loading was >5%. CUR NPs were stable for up to 5 months at 4°C and up to 96 hours at 37°C in PBS buffer (pH 7.4) without significant changes in particle sizes. For both sensitive and resistant PC3 and DU145 cell lines, CUR NPs significantly reduced IC50 values over free drug.

Conclusions (d):

We successfully prepare CUR NPs using lipid-based NPs. CUR NPs significantly improved cytotoxicity of CUR in sensitive and resistant prostate cancer cells compared to free CUR.