Abstract Title

The Effects of Androgens on COX2 signaling in Oxidatively Stressed Dopamine Neurons

Presenter Name

Shaletha Holmes

Abstract

Hypothesis: We hypothesize that under oxidative stress conditions, the androgen, testosterone, will increase COX2 induced alpha-synuclein expression, leading to apoptosis in dopamine neurons.

Materials and Methods: To test our hypothesis, we exposed a dopaminergic cell line (N27 cells) to a sublethal concentration of the pro-oxidant, tert-butyl hydrogen peroxide (H2O2) for 24 hours and assessed the role of testosterone on COX2 signaling.

Results: Under low oxidative stress conditions, COX2 protein levels are low and alpha-synuclein expression and apoptosis are absent. However, under oxidative stress conditions, COX2, alpha synuclein, and apoptosis were increased, and these factors were exacerbated by testosterone.

Conclusion: Our data shows that androgens may mediate the gender differences observed in PD by activating COX2 mediated inflammation and oxidative stress in dopamine neurons.

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The Effects of Androgens on COX2 signaling in Oxidatively Stressed Dopamine Neurons

Hypothesis: We hypothesize that under oxidative stress conditions, the androgen, testosterone, will increase COX2 induced alpha-synuclein expression, leading to apoptosis in dopamine neurons.

Materials and Methods: To test our hypothesis, we exposed a dopaminergic cell line (N27 cells) to a sublethal concentration of the pro-oxidant, tert-butyl hydrogen peroxide (H2O2) for 24 hours and assessed the role of testosterone on COX2 signaling.

Results: Under low oxidative stress conditions, COX2 protein levels are low and alpha-synuclein expression and apoptosis are absent. However, under oxidative stress conditions, COX2, alpha synuclein, and apoptosis were increased, and these factors were exacerbated by testosterone.

Conclusion: Our data shows that androgens may mediate the gender differences observed in PD by activating COX2 mediated inflammation and oxidative stress in dopamine neurons.