Departmental Affiliation and City, State, Zip for All Authors

Department of Biology, Montgomery, Alabama, 36104; Institute for Cardiovascular and Metabolic Disease, Fort Worth, Texas, 76107; Institute for Cardiovascular and Metabolic Disease, Fort Worth, Texas, 76107

Scientific Abstract

Despite substantial chemotherapeutic advances in the 21st century, toxicity remains a prevailing obstacle in cancer treatment. Previously, the Lacko Lab has shown that scavenger receptor B1 (SR-B1) overexpression is a hallmark of several cancers. The natural ligand of this receptor is circulating HDL, whose wildtype action is the receptor-mediated delivery of cholesterol in an apolipoprotein A1-dependent manner (1). In this study, a dual P13K/mTOR inhibitor was incorporated into reconstituted high density lipoprotein (rHDL) nanoparticles, and subsequently tested against a panel of glioblastoma multiforme (GBM) cell lines. The mean diameter of the nanoparticles were 15.7 nm with a standard deviation of 4.5 nm and a polydispersity index of 0.160, and drug concentration of 73.35 uM/mL. These nanoparticles provided an appreciable protective effect against astrocytes while having an IC50 value of 103 nM against GBM line LN229.

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DACTOLISIB-LOADED RECONSTITUTED HIGH DENSITY LIPOPROTEINS (RHDAL): A CANDIDATE FOR GLIOBLASTOMA THERAPY

Despite substantial chemotherapeutic advances in the 21st century, toxicity remains a prevailing obstacle in cancer treatment. Previously, the Lacko Lab has shown that scavenger receptor B1 (SR-B1) overexpression is a hallmark of several cancers. The natural ligand of this receptor is circulating HDL, whose wildtype action is the receptor-mediated delivery of cholesterol in an apolipoprotein A1-dependent manner (1). In this study, a dual P13K/mTOR inhibitor was incorporated into reconstituted high density lipoprotein (rHDL) nanoparticles, and subsequently tested against a panel of glioblastoma multiforme (GBM) cell lines. The mean diameter of the nanoparticles were 15.7 nm with a standard deviation of 4.5 nm and a polydispersity index of 0.160, and drug concentration of 73.35 uM/mL. These nanoparticles provided an appreciable protective effect against astrocytes while having an IC50 value of 103 nM against GBM line LN229.

Manuscript Number

1013