Date of Award

7-1-2008

Degree Type

Restricted Access Thesis

Degree Name

Master of Science

Field of Study

Biomedical Sciences

Department

Graduate School of Biomedical Sciences

First Advisor

Dan Dimitrijevich

Second Advisor

Ignacy Gryczynski

Third Advisor

Harlan Jones

Abstract

Arafeh, Reem B., Strategy to Design a Stratified Lamellar Corneal Stroma Construct. Master of Science (Biomedical Sciences), July 2nd, 2008, 103pp., 1 table, 42 illustrations, bibliography, 63 titles. Cornea is the transparent portion of the eye. Its three cellular compartments: the epithelium, stroma, and endothelium, are optimized for transparency and focusing. Although corneal grafting with donor corneas is a successful treatment to restore vision, there is an acute shortage of appropriate quality donor tissue. Tissue engineering is a new approach that addresses this shortage. In this thesis it was demonstrated that lamellar stromal structure is reproduced by stacking thin collagen films. Collagen fibril organization in the thin films directs cellular alignment allowing assembly of an orthogonal stroma construct that transmits more light than most other published constructs and is optically active. Whereas the cell alignment and lamellar arrangement were successful, optimization of optical properties is a major future goal.

Comments

Arafeh, Reem B., Strategy to Design a Stratified Lamellar Corneal Stroma Construct. Master of Science (Biomedical Sciences), July 2nd, 2008, 103pp., 1 table, 42 illustrations, bibliography, 63 titles. W 4.5 A658S 2008

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