Date of Award
Restricted Internship Practicum Report
Master of Science
Field of Study
Graduate School of Biomedical Sciences
Dr. Arthur Eisenberg
The project conducted at the Forensic Testing Laboratories (FTL) in Las Cruces, NM involved an internal validation of the AmpFlSTR® Yfiler™ PCR Amplification kit. FTL currently implements the PowerPlex® Y kit which amplifies eleven Y-STR markers. The Yfiler™ kit provides more discriminatory power as it contains sixteen Y-STR markers. The validation included sensitivity, mixture, case type samples, contamination, precision and injection time change studies as required by the National Standards. Based on the data obtained, the Yfiler™ kit successfully detects male DNA in the presence of female DNA, and the correct number of male contributors in male/male mixtures is distinguishable. The additional loci included in the Yfiler™ kit can provide more insight than what is achieved through the PowerPlex® Y kit.
"Internal Validation of the AmpFlSTR® Yfiler™ PCR Amplification Kit" Fort Worth, Tx: University of North Texas Health Science Center;