Date of Award

5-1-2004

Degree Type

Restricted Access Professional Report

Degree Name

Master of Science

Field of Study

Biotechnology

Department

Graduate School of Biomedical Sciences

First Advisor

Julian Borejdo

Second Advisor

Walter J. McConathy

Third Advisor

Maya P. Nair

Abstract

The purpose of this study was to perform uptake and efflux studies of ORB in drug-sensitive (OV1063) and drug-resistant (SK-OV-3 and OVCAR-3) cells. Experiments were carried out by delivering ORB to the cells with and without rHDL to compare the amount of ORB taken up and expelled by the respective cells. Because serum contains lipoproteins, the experiments were also done in the absence of serum to show the specific effect of the rHDL vehicle. Because the efflux pumps responsible for multi-drug resistance are overexpressed and amplified in resistant cells, the efflux of ORB should be higher in these cells compared to the drug-sensitive cell line, OV1063. But, since our rHDL system is designed to reduce drug resistance by releasing the drug directly inside the cell bypassing the efflux pumps, the efflux of ORB should be less when it is delivered with rHDL.

Comments

W 4.8 B989E 2004

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