Date of Award

5-1-2014

Degree Type

Thesis

Degree Name

Master of Science

Field of Study

Forensic Genetics

First Advisor

Bruce Budowle

Abstract

DNA typing can be a labor intensive and time-consuming process which, even in ideal situations, can take up to approximately one full day from collected swab to generated genotype profile. To expedite the process from sample-to-result, automated rapid DNA typing platforms have been developed. These turnkey systems offer the potential of reducing the time from reference sample collection to fully-interpreted profile in less than two hours. However, for full consideration of rapid technology, results should be of equal quality to that of currently accepted methodologies. This project tested the hypothesis that automated rapid STR genotyping platforms perform as well as standard STR typing methods for typing reference samples by comparing the ability of NetBio’s DNAscan™ automated rapid STR genotyping platform (NetBio, Inc., Waltham, MA) and standard STR typing methods to generate DNA profiles from reference samples. Multiple buccal swabs, collected from a number of individuals, were analyzed with the DNAscan instrument, an automated rapid STR genotyping platform that utilizes PowerPlex® 16 chemistry (Promega Corporation, Madison, WI). The rapid STR results were comparable with those obtained from the same individuals by standard STR genotyping processes also employing PowerPlex® 16 chemistry.

Comments

Moore, Andrea B., STR Typing of Reference Samples with Rapid DNA Technology. Master of Science (Forensic Genetics), May, 2014, 62 pp., 3 tables, 17 illustrations, references, 30 titles. Available May 2015

Share

COinS