Date of Award

8-1-2008

Degree Type

Restricted Access Professional Report

Degree Name

Master of Science

Field of Study

Cell Biology and Genetics

Department

Graduate School of Biomedical Sciences

First Advisor

Arthur Eisenberg

Second Advisor

Joseph Warren

Third Advisor

John Planz

Abstract

Klein, Cindi Leann. Internal Validation of Applied Biosystems Quantifiler Duo DNA Quantification Kit for Use in Forensic Casework. Master of Science (Forensic Genetics), August 2008, 48 pages, 19 figures, 4 tables, 10 references. Applied Biosystems (Foster City, CA) has recently developed a new DNA quantification kit, Quantifiler Duo, which simultaneously quantitates the total amount of amplifiable human and male DNA present in a sample. The kit is a multiplex real-time PCR system based on the 5’ nuclease assay and Taqman probe-based technology. An internal validation was performed at the Harris County Medical Examiner’s Office using Quantifiler Duo and was comprised of six studies: sensitivity, reproducibility, cross-contamination, precision, mixture, and stability. The preliminary results show an increased sensitivity from the currently used Quantifiler Human and Quantifiler Y Human Male DNA Quantification kits. The accuracy and precision of the Quantifiler Duo kit was also comparable or superior to that of the current kits.

Comments

Klein, Cindi Leann. Internal Validation of Applied Biosystems Quantifiler Duo DNA Quantification Kit for Use in Forensic Casework. Master of Science (Forensic Genetics), August 2008, 48 pages, 19 figures, 4 tables, 10 references. W 4.8 K64I 2008

Share

COinS