Date of Award

5-1-2015

Degree Type

Thesis

Degree Name

Master of Science

Field of Study

Forensic Genetics

Department

Graduate School of Biomedical Sciences

First Advisor

Arthur J. Eisenberg

Second Advisor

Rhonda Roby

Third Advisor

John V. Planz

Abstract

Cell line authentication is an essential step in ensuring the integrity and reproducibility of biomedical research. The major contaminants in cell cultures are fungi, viruses, bacteria and contamination from other cell lines of the same or different species. Contaminants alter the physiology and properties of cells, compromising the results of experiments. In this study, an improved multiplex assay was developed, detecting mycoplasma and mouse cell line contamination, while performing DNA typing.

The assay was tested on cell cultures, the reproducibility of the assay was verified, sample collection and procedures were optimized and limit of detection for contaminants were determined. A survey was conducted to assess the interest in an in-house cell line authentication and contamination assessment service.

Comments

Ormos, Andrea. Cell line authentication and contamination assessment for human cell cultures. Master of Science (Forensic Genetics), May 2015, 82 pp., 7 tables, 25 figures, 33 references.