Date of Award

8-1-2015

Degree Type

Thesis

Degree Name

Master of Science

Field of Study

Forensic Genetics

Department

Graduate School of Biomedical Sciences

First Advisor

Arthur J. Eisenberg

Second Advisor

Joseph E. Warren

Third Advisor

Raghu R. Krishnamoorthy

Abstract

Genetic results from bone samples often yield low quantities of DNA and poor quality of genetic data. Proteinase K is a proteinase that is commonly used in DNA extraction methods, however the target proteins of Proteinase K do not closely align with the makeup of bone. Collagenase Type 2 is a protease that is more specific to the breakdown of collagen, which bone is comprised of. This study looked at the potential effects of Collagenase Type 2 digestion on bone samples compared to the effects of Proteinase K on quantity and quality of genetic typing. This study also incorporates the EZ1 Advanced XL purification platform and the AmpFLSTR Globalfiler Amplification Kit.

Comments

Barrett, Lisa C. Effect of Collagenase Type 2 and Proteinase K Digestion on DNA Yield from Bone Samples Purified on the EZ1 Advanced XL. Master of Science (Biomedical Sciences, Forensic Genetics). July 2015. 39 pp., 16 Figures, 20 References. Available worldwide August 2016.