Date of Award

8-1-2016

Degree Type

Thesis

Degree Name

Master of Science

Field of Study

Forensic Genetics

Department

Graduate School of Biomedical Sciences

First Advisor

Joseph E. Warren

Second Advisor

Bobby L. LaRue

Third Advisor

Lisa Hodge

Abstract

Formalin-fixed, paraffin embedded (FFPE) tissue samples are often the only sample type available for testing in pathological and clinical fields. Obtaining usable DNA from FFPE samples is difficult due to the formalin fixation method. Formaldehyde, a large component of formalin, causes DNA-protein cross-linking as well as other issues that must be overcome to obtain useable DNA. Many methods for deparaffinization exist to reduce the issues that arise when working with FFPE samples. This project focuses on comparing two methods of deparaffinization: a method described by Bosso and Al-Mulla and a method used by the University of North Texas Center For Human Identification (UNTCHI), to determine if there is a significant difference in DNA obtained from FFPE samples.

Comments

Leonard, Raechal. Comparison of Deparaffinization Methods With DNA Quantification. Master of Science (Biomedical Sciences, Forensic Genetics). August 2016. 29 pp., 13 Tables, 12 Figures, 22 References.

Share

COinS