Presentation Title (IN ALL CAPS)

SURFACE CHARGE AFFECTS CELLULAR UPTAKE AND INTRACELLULAR DISTRIBUTION OF HYDROPHILIC DRUG LOADED NANOPARTICLES FOR CANCER THERAPY

Departmental Affiliation and City, State, Zip for All Authors

Department of Microbiology, Immunology & Genetics, UNT Health Science Center, Fort Worth TX 76107

Scientific Abstract

Abstract Nanoparticles (NPs) are submicroscopic particles (< 200 nm) that encapsulate anti-cancer drugs and transport them to specific cells in the human body. Targeting ligands can be employed to increase the specificity of the NP and to prevent off-target side effects. Additionally, physical properties such as size and zeta potential (ZP) (surface charge), can facilitate the localization and distribution of NPs in the cells and improve overall pharmacodynamic efficiency. Tumors develop an immature vasculature with a leaky formation that enables the NPs to pass into tumors and concentrate more drug in the affected areas. Cell uptake, a critical component in the effective delivery of cancer therapeutics, is affected by the ZP. (Khine, et al., 2016) This surface charge also affects internal localization, cytotoxicity, mechanism of cellular toxicity, and targeting propensity. (Frohlich, E., 2012) Polymeric and liposomal NPs are the two most common types of NPs (Zhang, et al., 2008). Poly (lactic-co-glycolic acid) (PLGA) NPs were used to encapsulate the hydrophilic drug, doxorubicin hydrochloride (DOX). The overall surface charge, size, and encapsulation efficiency were recorded to determine what surface charge of the nanoparticle results in the highest distribution of DOX within the cells.

This document is currently not available here.

Share

COinS
 

SURFACE CHARGE AFFECTS CELLULAR UPTAKE AND INTRACELLULAR DISTRIBUTION OF HYDROPHILIC DRUG LOADED NANOPARTICLES FOR CANCER THERAPY

Abstract Nanoparticles (NPs) are submicroscopic particles (< 200 nm) that encapsulate anti-cancer drugs and transport them to specific cells in the human body. Targeting ligands can be employed to increase the specificity of the NP and to prevent off-target side effects. Additionally, physical properties such as size and zeta potential (ZP) (surface charge), can facilitate the localization and distribution of NPs in the cells and improve overall pharmacodynamic efficiency. Tumors develop an immature vasculature with a leaky formation that enables the NPs to pass into tumors and concentrate more drug in the affected areas. Cell uptake, a critical component in the effective delivery of cancer therapeutics, is affected by the ZP. (Khine, et al., 2016) This surface charge also affects internal localization, cytotoxicity, mechanism of cellular toxicity, and targeting propensity. (Frohlich, E., 2012) Polymeric and liposomal NPs are the two most common types of NPs (Zhang, et al., 2008). Poly (lactic-co-glycolic acid) (PLGA) NPs were used to encapsulate the hydrophilic drug, doxorubicin hydrochloride (DOX). The overall surface charge, size, and encapsulation efficiency were recorded to determine what surface charge of the nanoparticle results in the highest distribution of DOX within the cells.

Manuscript Number

1042