Presentation Title (IN ALL CAPS)

VIRTUAL DISSECTION OF COMPLEX MUSCLES WITH DICECT

Departmental Affiliation and City, State, Zip for All Authors

Department of Anthropology, Texas State University, San Marcos, TX, 78666, and Summer Opportunities in Anatomy Research Program, Center for Anatomical Sciences, Fort Worth, TX, 76107, UNTHSC; Center for Anatomical Sciences, UNTHSC, Fort Worth, TX, 76107

Scientific Abstract

Computed tomography (CT) scanning is commonly used in biomedical research to image the skeleton. However, the boundaries of soft tissues such as muscle and cartilage are not well defined in CT scans. Here we demonstrate how diffusible iodine-based contrast-enhanced computed tomography (diceCT) can be used to visualize soft tissues and measure muscle volumes more accurately than in traditional dissection methods, particularly in small rodent species. The goal of this project is to assess changes in the growth trajectories of masticatory musculature due to variation in the biomechanical properties of diet. Male Sprague-Dawley rats were raised from weaning to adulthood (12 weeks), and randomly separated into four hard and/or soft dietary treatment groups. Post-sacrifice, cranial tissues were fixed in 4% PFM for 36 hours and stored in 70% EA at 4℃. Specimens were stained in 11.25% Lugol’s solution (I2KI) for 48 hours and imaged in a Skyscan 1172 micro-CT scanner (0.018 mm3 voxels). Masticatory muscles were manually segmented every 10 slices using the Segment Editor module in 3D Slicer. The “Fill Between Slices” function was used to generate complete volumes which were measured by the Volumetric Statistics module. Preliminary results suggest that, contrary to our expectations, animals raised on soft diets have larger temporalis and superficial masseter muscles. DiceCT is a promising methoding for soft tissue analysis that complements CT analyses of bone. Volumetric and geometric data can be obtained for small and/or complex musculature where limitations exist for traditional dissection methods.

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VIRTUAL DISSECTION OF COMPLEX MUSCLES WITH DICECT

Computed tomography (CT) scanning is commonly used in biomedical research to image the skeleton. However, the boundaries of soft tissues such as muscle and cartilage are not well defined in CT scans. Here we demonstrate how diffusible iodine-based contrast-enhanced computed tomography (diceCT) can be used to visualize soft tissues and measure muscle volumes more accurately than in traditional dissection methods, particularly in small rodent species. The goal of this project is to assess changes in the growth trajectories of masticatory musculature due to variation in the biomechanical properties of diet. Male Sprague-Dawley rats were raised from weaning to adulthood (12 weeks), and randomly separated into four hard and/or soft dietary treatment groups. Post-sacrifice, cranial tissues were fixed in 4% PFM for 36 hours and stored in 70% EA at 4℃. Specimens were stained in 11.25% Lugol’s solution (I2KI) for 48 hours and imaged in a Skyscan 1172 micro-CT scanner (0.018 mm3 voxels). Masticatory muscles were manually segmented every 10 slices using the Segment Editor module in 3D Slicer. The “Fill Between Slices” function was used to generate complete volumes which were measured by the Volumetric Statistics module. Preliminary results suggest that, contrary to our expectations, animals raised on soft diets have larger temporalis and superficial masseter muscles. DiceCT is a promising methoding for soft tissue analysis that complements CT analyses of bone. Volumetric and geometric data can be obtained for small and/or complex musculature where limitations exist for traditional dissection methods.

Manuscript Number

1031